Ambrosio may refer to the following:
- Alessandra Ambrosio - a Brazilian model
- Franco Ambrosio (1932-2009) - an Italian businessman and sponsor of Formula One teams in the 1970s
- Giovanni Ambrosio - a 15th-century Italian dancer and writer on dance (Guglielmo Ebreo da Pesaro)
- Luigi Ambrosio - mathematician
- Vittorio Ambrosio - an Italian general from World War I and World War II
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