Separator can refer to:
- Cream separator, separates cream from milk
- Community separator, a term of urban planning
- Separator (oil production), of an oil production plant
- Vapor-liquid separator, separates a vapor-liquid mixture
- Demister (vapor), removal of liquid droplets entrained in a vapor stream
- Separator (electricity), a porous or ion-conducting barrier used to separate anode and cathode in electrochemical systems, also known as diaphragm
- Planar separator theorem, a theorem in graph theory
- Vertex separator, a notion in graph theory
- A mathematical sign used to separate the integer part from the fractional part of a number. For example, the decimal separator and the binary separator
- A synonym of delimiter in computer parlance
- Orthodontic spacer, also known as orthodontic separators
- Four of the C0 and C1 control codes used in digital character encoding