The overlap extension polymerase chain reaction (or OE-PCR) is a variant of PCR. It is also referred to as Splicing by overlap extension / Splicing by overhang extension (SOE) PCR. It is used to insert specific mutations at specific points in a sequence or to splice smaller DNA fragments into a larger polynucleotide.
Read more about Overlap Extension Polymerase Chain Reaction: Splicing of DNA Molecules, Introduction of Mutations
Famous quotes containing the words overlap, extension, chain and/or reaction:
“A lady is nothing very specific. One man’s lady is another man’s woman; sometimes, one man’s lady is another man’s wife. Definitions overlap but they almost never coincide.”
—Russell Lynes (b. 1910)
“We are now a nation of people in daily contact with strangers. Thanks to mass transportation, school administrators and teachers often live many miles from the neighborhood schoolhouse. They are no longer in daily informal contact with parents, ministers, and other institution leaders . . . [and are] no longer a natural extension of parental authority.”
—James P. Comer (20th century)
“Oh yes, that’s right. They chain up wild animals. That’s all I am, an animal.”
—John Elder [Anthony Hinds], British screenwriter, and Terence Fisher. Leon (Oliver Reed)
“In a land which is fully settled, most men must accept their local environment or try to change it by political means; only the exceptionally gifted or adventurous can leave to seek his fortune elsewhere. In America, on the other hand, to move on and make a fresh start somewhere else is still the normal reaction to dissatisfaction and failure.”
—W.H. (Wystan Hugh)