The overlap extension polymerase chain reaction (or OE-PCR) is a variant of PCR. It is also referred to as Splicing by overlap extension / Splicing by overhang extension (SOE) PCR. It is used to insert specific mutations at specific points in a sequence or to splice smaller DNA fragments into a larger polynucleotide.
Read more about Overlap Extension Polymerase Chain Reaction: Splicing of DNA Molecules, Introduction of Mutations
Famous quotes containing the words overlap, extension, chain and/or reaction:
“A lady is nothing very specific. One man’s lady is another man’s woman; sometimes, one man’s lady is another man’s wife. Definitions overlap but they almost never coincide.”
—Russell Lynes (b. 1910)
“Slavery is founded in the selfishness of man’s nature—opposition to it, is [in?] his love of justice.... Repeal the Missouri compromise—repeal all compromises—repeal the declaration of independence—repeal all past history, you still can not repeal human nature. It still will be the abundance of man’s heart, that slavery extension is wrong; and out of the abundance of his heart, his mouth will continue to speak.”
—Abraham Lincoln (1809–1865)
“You know, he wanted to shoot the Royal Family, abolish marriage, and put everybody who’d been to public school in a chain gang. Yeah, he was a idealist, your dad was.”
—David Mercer, British screenwriter, and Karel Reisz. Mrs. Dell (Irene Handl)
“More and more, when faced with the world of men, the only reaction is one of individualism. Man alone is an end unto himself. Everything one tries to do for the common good ends in failure.”
—Albert Camus (1913–1960)