Overlap Extension Polymerase Chain Reaction

The overlap extension polymerase chain reaction (or OE-PCR) is a variant of PCR. It is also referred to as Splicing by overlap extension / Splicing by overhang extension (SOE) PCR. It is used to insert specific mutations at specific points in a sequence or to splice smaller DNA fragments into a larger polynucleotide.

Read more about Overlap Extension Polymerase Chain Reaction:  Splicing of DNA Molecules, Introduction of Mutations

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