The overlap extension polymerase chain reaction (or OE-PCR) is a variant of PCR. It is also referred to as Splicing by overlap extension / Splicing by overhang extension (SOE) PCR. It is used to insert specific mutations at specific points in a sequence or to splice smaller DNA fragments into a larger polynucleotide.
Read more about Overlap Extension Polymerase Chain Reaction: Splicing of DNA Molecules, Introduction of Mutations
Famous quotes containing the words overlap, extension, chain and/or reaction:
“A lady is nothing very specific. One man’s lady is another man’s woman; sometimes, one man’s lady is another man’s wife. Definitions overlap but they almost never coincide.”
—Russell Lynes (b. 1910)
“Slavery is founded on the selfishness of man’s nature—opposition to it on his love of justice. These principles are in eternal antagonism; and when brought into collision so fiercely as slavery extension brings them, shocks and throes and convulsions must ceaselessly follow.”
—Abraham Lincoln (1809–1865)
“Man ... cannot learn to forget, but hangs on the past: however far or fast he runs, that chain runs with him.”
—Friedrich Nietzsche (1844–1900)
“The excessive increase of anything often causes a reaction in the opposite direction.”
—Plato (c. 427–347 B.C.)