Overlap Extension Polymerase Chain Reaction

The overlap extension polymerase chain reaction (or OE-PCR) is a variant of PCR. It is also referred to as Splicing by overlap extension / Splicing by overhang extension (SOE) PCR. It is used to insert specific mutations at specific points in a sequence or to splice smaller DNA fragments into a larger polynucleotide.

Read more about Overlap Extension Polymerase Chain Reaction:  Splicing of DNA Molecules, Introduction of Mutations

Famous quotes containing the words overlap, extension, chain and/or reaction:

    A lady is nothing very specific. One man’s lady is another man’s woman; sometimes, one man’s lady is another man’s wife. Definitions overlap but they almost never coincide.
    Russell Lynes (b. 1910)

    ‘Tis the perception of the beautiful,
    A fine extension of the faculties,
    Platonic, universal, wonderful,
    Drawn from the stars, and filtered through the skies,
    Without which life would be extremely dull.
    George Gordon Noel Byron (1788–1824)

    It is the future that creates his present.
    All is an interminable chain of longing.
    Robert Frost (1874–1963)

    Christianity was only a very strong and singularly well-timed Salvation Army movement that happened to receive help from an unusual and highly dramatic incident. It was a Puritan reaction in an age when, no doubt, a Puritan reaction was much wanted; but like all sudden violent reactions, it soon wanted reacting against.
    Samuel Butler (1835–1902)