The overlap extension polymerase chain reaction (or OE-PCR) is a variant of PCR. It is also referred to as Splicing by overlap extension / Splicing by overhang extension (SOE) PCR. It is used to insert specific mutations at specific points in a sequence or to splice smaller DNA fragments into a larger polynucleotide.
Read more about Overlap Extension Polymerase Chain Reaction: Splicing of DNA Molecules, Introduction of Mutations
Famous quotes containing the words overlap, extension, chain and/or reaction:
“A lady is nothing very specific. One mans lady is another mans woman; sometimes, one mans lady is another mans wife. Definitions overlap but they almost never coincide.”
—Russell Lynes (b. 1910)
“Slavery is founded in the selfishness of mans natureopposition to it, is [in?] his love of justice.... Repeal the Missouri compromiserepeal all compromisesrepeal the declaration of independencerepeal all past history, you still can not repeal human nature. It still will be the abundance of mans heart, that slavery extension is wrong; and out of the abundance of his heart, his mouth will continue to speak.”
—Abraham Lincoln (18091865)
“A chain is no stronger than its weakest link, and life is after all a chain.”
—William James (18421910)
“The excessive increase of anything often causes a reaction in the opposite direction.”
—Plato (c. 427347 B.C.)