The overlap extension polymerase chain reaction (or OE-PCR) is a variant of PCR. It is also referred to as Splicing by overlap extension / Splicing by overhang extension (SOE) PCR. It is used to insert specific mutations at specific points in a sequence or to splice smaller DNA fragments into a larger polynucleotide.
Read more about Overlap Extension Polymerase Chain Reaction: Splicing of DNA Molecules, Introduction of Mutations
Famous quotes containing the words overlap, extension, chain and/or reaction:
“A lady is nothing very specific. One man’s lady is another man’s woman; sometimes, one man’s lady is another man’s wife. Definitions overlap but they almost never coincide.”
—Russell Lynes (b. 1910)
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Without which life would be extremely dull.”
—George Gordon Noel Byron (1788–1824)
“The name of the town isn’t important. It’s the one that’s just twenty-eight minutes from the big city. Twenty-three if you catch the morning express. It’s on a river and it’s got houses and stores and churches. And a main street. Nothing fancy like Broadway or Market, just plain Broadway. Drug, dry good, shoes. Those horrible little chain stores that breed like rabbits.”
—Joseph L. Mankiewicz (1909–1993)
“An actor must communicate his author’s given message—comedy, tragedy, serio- comedy; then comes his unique moment, as he is confronted by the looked-for, yet at times unexpected, reaction of the audience. This split second is his; he is in command of his medium; the effect vanishes into thin air; but that moment has a power all its own and, like power in any form, is stimulating and alluring.”
—Eleanor Robson Belmont (1878–1979)