The overlap extension polymerase chain reaction (or OE-PCR) is a variant of PCR. It is also referred to as Splicing by overlap extension / Splicing by overhang extension (SOE) PCR. It is used to insert specific mutations at specific points in a sequence or to splice smaller DNA fragments into a larger polynucleotide.
Read more about Overlap Extension Polymerase Chain Reaction: Splicing of DNA Molecules, Introduction of Mutations
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“A lady is nothing very specific. One man’s lady is another man’s woman; sometimes, one man’s lady is another man’s wife. Definitions overlap but they almost never coincide.”
—Russell Lynes (b. 1910)
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—Abraham Lincoln (1809–1865)
“A chain is no stronger than its weakest link, and life is after all a chain.”
—William James (1842–1910)
“The excessive increase of anything often causes a reaction in the opposite direction.”
—Plato (c. 427–347 B.C.)