Applications of Spontaneous Isopeptide Bond Formation
Recently, researchers have exploited spontaneous isopeptide bond formation to develop a peptide tag called SpyTag. SpyTag can spontaneously and irreversibly react with its binding partner (a protein termed SpyCatcher) through a covalent isopeptide bond. This molecular tool may have applications for in vivo protein targeting, fluorescent microscopy, and irreversible attachment for a protein microarray.
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