An isopeptide bond is an amide bond that is not present on the main chain of a protein. The bond forms between the carboxyl terminus of one protein and the amino group of a lysine residue on another (target) protein.
Isopeptide bonds can occur between the side chain amine of lysine and the side chain carboxyl groups of either glutamate or aspartate. Bond formation can be either enzyme catalyzed, as in the case for the bond formed between lysine and glutamine catalyzed by transglutaminases, or it can form spontaneously as observed in HK97 bacteriophage capsid formation and Gram-positive bacterial pili. Spontaneous isopeptide bond formation requires the presence of another residue, glutamic acid, which catalyzes bond formation in a proximity induced manner.
An example of a small peptide containing an isopeptide bond is glutathione, which has a bond between the side chain of a glutamate residue and the amino group of a cysteine residue. An example of a protein involved in isopeptide bonding is ubiquitin, which gets attached to other proteins with a bond between the C-terminal glycine residue of ubiquitin and a lysine side chain of the substrate protein.
Read more about Isopeptide Bond: Biological Roles of Isopeptide Bonds: Signalling and Structural, Applications of Spontaneous Isopeptide Bond Formation
Famous quotes containing the word bond:
“It may comfort you to know that if your child reaches the age of eleven or twelve and you have a good bond or relationship, no matter how dramatic adolescence becomes, you children will probably turn out all right and want some form of connection to you in adulthood.”
—Charlotte Davis Kasl (20th century)