Generation of Loops in DsDNA
A single molecule study using atomic force microscopy (AFM) and tethered particle motion (TPM) has observed that ISWI can bound naked DNA in absence ATP wrapping DNA around the protein. In presence of ATP, the protein generates DNA loops while simultaneously generating negative supercoils in the template. The first figure in this paper shows three AFM images from where single DNA interacting with ISWI was deposed on mica surfaces. On the center, a single ISWI is bound near the end of a dsDNA template. The right image shows two DNA loops generated by ISWI. These loops contains supercoils. The TPM study instead showed that the duration of loop formed by ISWI was ATP-dependent.
Read more about this topic: Imitation SWI
Famous quotes containing the words generation of, generation and/or loops:
“There may perhaps be a new generation of doctors horrified by lacerations, infections, women who have douched with kitchen cleanser. What an irony it would be if fanatics continued to kill and yet it was the apathy and silence of the medical profession that most wounded the ability to provide what is, after all, a medical procedure.”
—Anna Quindlen (b. 1952)
“One of the things that is most striking about the young generation is that they never talk about their own futures, there are no futures for this generation, not any of them and so naturally they never think of them. It is very striking, they do not live in the present they just live, as well as they can, and they do not plan. It is extraordinary that whole populations have no projects for a future, none at all.”
—Gertrude Stein (1874–1946)
“An accurate charting of the American woman’s progress through history might look more like a corkscrew tilted slightly to one side, its loops inching closer to the line of freedom with the passage of time—but like a mathematical curve approaching infinity, never touching its goal. . . . Each time, the spiral turns her back just short of the finish line.”
—Susan Faludi (20th century)