The protein ISWI or imitation SWI of drosophila melanogaster (common fruit fly), is the first ATPase subunit which has been isolated in the ISWI chromatin remodeling family. This protein presents high level of similarity to the SWI/SNF chromatin remodeling family in the ATPase domain. Outside the ATPase domain ISWI loses the similarity with the member of the SWI/SNF family, possessing a SANT domain instead of the bromodomain. The protein ISWI can interact with several proteins giving three different chromatin-remodeling complexes in Drosophila melanogaster: NURF(nucleosome remodeling factor), CHRAC (chromatin remodeling and assembly complex) and ACF (ATP-utilising chromatin remodeling and assembly Factor).
In vitro, the ISWI protein alone can assemble nucleosomes on linear DNA and it can move nucleosomes on linear DNA from the center to the extremities. Inside the CHRAC complex, ISWI catalyzes the inverse reaction, moving nucleosomes from the extremities to the center.
Read more about Imitation SWI: Generation of Loops in DsDNA
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