Holtby is a small village and civil parish in the unitary authority of the City of York in North Yorkshire, England. It lies on the A166 about 5 miles (8 km) east of York.
According to the 2001 census the parish had a population of 152. Prior to 1996 it had been part of the Ryedale district.
Holtby was served by Holtby railway station on the York to Beverley Line between 1847 and 1939.