Hlushko or Glushko (Ukrainian: Глушко) is a Ukrainian surname, that may refer to :
- Glushko (crater), a young impact crater on the Moon
- Julia Glushko (born 1990), a right-handed Israeli tennis player
- Valentin Glushko (1908-1989), a Soviet engineer
- Yuri Hlushko-Mova (1882-1942), a Ukrainian public and political figure
- 6357 Glushko, a Main-belt Asteroid