An expressed sequence tag or EST is a short sub-sequence of a cDNA sequence. They may be used to identify gene transcripts, and are instrumental in gene discovery and gene sequence determination. The identification of ESTs has proceeded rapidly, with approximately 72.6 million ESTs now available in public databases (e.g. GenBank 11 May 2011, all species).
An EST results from one-shot sequencing of a cloned cDNA. The cDNAs used for EST generation are typically individual clones from a cDNA library. The resulting sequence is a relatively low quality fragment whose length is limited by current technology to approximately 500 to 800 nucleotides. Because these clones consist of DNA that is complementary to mRNA, the ESTs represent portions of expressed genes. They may be represented in databases as either cDNA/mRNA sequence or as the reverse complement of the mRNA, the template strand.
ESTs can be mapped to specific chromosome locations using physical mapping techniques, such as radiation hybrid mapping, Happy mapping, or FISH. Alternatively, if the genome of the organism that originated the EST has been sequenced, one can align the EST sequence to that genome using a computer.
The current understanding of the human set of genes (as of 2006) includes the existence of thousands of genes based solely on EST evidence. In this respect, ESTs have become a tool to refine the predicted transcripts for those genes, which leads to the prediction of their protein products and ultimately their function. Moreover, the situation in which those ESTs are obtained (tissue, organ, disease state - e.g. cancer) gives information on the conditions in which the corresponding gene is acting. ESTs contain enough information to permit the design of precise probes for DNA microarrays that then can be used to determine the gene expression.
Some authors use the term "EST" to describe genes for which little or no further information exists besides the tag.
The significance of ESTs, their properties, methods to analyze EST dataset and their applications in different areas of biology have been reviewed by Nagaraj et al. (2007).
Famous quotes containing the words expressed, sequence and/or tag:
“Nothing is poorer than a truth expressed as it was thought. Committed to writing in such cases, it is not even a bad photograph.... Truth wants to be startled abruptly, at one stroke, from her self-immersion, whether by uproar, music or cries for help.”
—Walter Benjamin (1892–1940)
“Reminiscences, even extensive ones, do not always amount to an autobiography.... For autobiography has to do with time, with sequence and what makes up the continuous flow of life. Here, I am talking of a space, of moments and discontinuities. For even if months and years appear here, it is in the form they have in the moment of recollection. This strange form—it may be called fleeting or eternal—is in neither case the stuff that life is made of.”
—Walter Benjamin (1892–1940)
“I believe in the flesh and the appetites,
Seeing, hearing, feeling, are miracles, and each part and tag of me
is a miracle.
Divine am I inside and out, and I make holy whatever I touch or am touch’d from,
The scent of these arm-pits aroma finer than prayer,
This head more than churches, bibles, and all the creeds.”
—Walt Whitman (1819–1892)