DNE may refer to:
- The convention of circling important information (such as URLs, or assignments) and marking it DNE (Short for do not erase) on chalkboards in academic institutions with shared lecture facilities.
- In mathematics it may be used as an abbreviation to illustrate that a proper solution to some problem Does Not Exist.
- An abbreviation for "Did not enter" or "Do not enter"
- The National Livestock Department (Direction Nationale d'Elevage - DNE) of Mauritania, which manages different aspects of agriculture in that nation
- Defining New Elegance - an English urban catchphrase by Alexander Hunter-Heslop, an international lifestyle critic.