Evidence of This Mechanism in-vivo
In vivo, deamination of the α-amino group of amino acids produces small amounts of ammonia. Researchers found that protein-incorporated allysine, (deaminated lysine) from bovine ligamentum nuchae elastin fibers appeared to be pyridine cross-linked. The structures of these cross-linked amino acids had 3,4,5- and 2,3,5-trisubstituted pyridine skeletons, specifically pyridinated desmosine (DESP) and pyridinated isodesmosine (IDP).
Extrapolating from an in-vitro elastin model under physiological conditions, the researchers found that the ratios of IDP to DESP corresponded extremely closely with values based on both a calculation of a theoretical Chichibabin pyridine synthesis of 3 mol allysine and 1 mol ammonia, and reported ratios of 2,3,5- to 3,4,5-trisubstituted pyridine ratios of a Chichibabin pyridine synthesis involving phenylacetaldehyde. They concluded with relative certainty that the pyridine cross-links found in elastin were, in fact, due to an in-vitro Chichibabin pyridine synthesis of ammonia and allysine.
Read more about this topic: Chichibabin Pyridine Synthesis
Famous quotes containing the words evidence of, evidence and/or mechanism:
“Generally there is no consistent evidence of significant differences in school achievement between children of working and nonworking mothers, but differences that do appear are often related to maternal satisfaction with her chosen role, and the quality of substitute care.”
—Ruth E. Zambrana, U.S. researcher, M. Hurst, and R.L. Hite. “The Working Mother in Contemporary Perspectives: A Review of Literature,” Pediatrics (December 1979)
“We perceive that the schemers return again and again to common sense and labor. Such is the evidence of history.”
—Henry David Thoreau (1817–1862)
“The law isn’t justice. It’s a very imperfect mechanism. If you press exactly the right buttons and are also lucky, justice may show up in the answer. A mechanism is all the law was ever intended to be.”
—Raymond Chandler (1888–1959)