Arlene may refer to:
- Arlene (Garfield), a fictional character in Garfield
- "Arlene" (song), the 1985 debut single by American country music artist Marty Stuart
- Arlene, a Beanie Baby cat produced by Ty, Inc.
- Arlene Francis
- Arlene Dahl
- Arlene Golonka
- Arlene Phillips, professional dancer and theatre choreographer
- Arlene Semeco, Venezuelan freestyle swimmer
- Arlene Xavier
- Arlene (The X-Files), a fictional character in The X-Files
- Hurricane Arlene (disambiguation)
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