AOT may refer to:
- AOT, the United States Navy hull classification symbol for "oil transporter"
- Aeronautical operations technician
- Airports of Thailand, an airport operator
- Ahead of Time, see AOT compiler
- Aosta Airport, Italy (IATA code)
- A.O. Trikala, a football club in Trikala, Greece
- Army of Two, a video game
- Aerosol OT, a common chemical reagent, see Dioctyl sodium sulfonsuccinate
- "Arse over Tit" a slang expression meaning "head over heels"
- Autorité organisatrice de transports, a French legal structure for the provision of public transport