Walkerville is a name given to several places:
- Australia
- Town of Walkerville, a local government area in South Australia
- Walkerville, South Australia, a suburb in north-eastern Adelaide
- Walkerville, Victoria, a town in southwest Gippsland
- Canada
- Walkerville, Ontario, a former company town that grew around the distillery built by Hiram Walker
- South Africa
- Walkerville, Gauteng
- United Kingdom
- Walkerville, North Yorkshire, in England
- Walkerville, Newcastle Upon Tyne, in England
- United States
- Walkerville, Michigan
- Walkerville, Montana
- In education
- Walkerville Collegiate Institute, a high school in Windsor, Ontario, Canada
- In entertainment
- Walkerville (fictional), the setting of the Magic School Bus cartoon series