TVO is an abbreviation with several different significations:
- Televisora de Oriente, a Venezuelan TV station
- Teollisuuden Voima, a Finnish nuclear power company
- Total Value of Ownership, an evaluation method
- TVOntario, a Canadian TV station
- Óptima Televisión, a Chilean TV station
- TV Oberfranken, a regional TV station in Upper Franconia, Germany
- TV Osaka, a Japanese TV station
- Tractor vaporising oil, engine fuel for British tractors
- Total viable organism, a microbiology term for determining the microbial content of a sample