Tuolumne may refer to:
- Tuolumne River, one of the major rivers draining the western slope Sierra Nevada mountains
- Tuolumne Meadows, in the eastern section of Yosemite National Park
- Grand Canyon of the Tuolumne, also in Yosemite National Park
- Tuolumne County, California, located in the Sierra Nevada
- Tuolumne City, California, an unincorporated community in Tuolumne County