Discovery of TmRNA and Early Work
tmRNA was first designated 10Sa RNA after a mixed “10S” electrophoretic fraction of Escherichia coli RNA was further resolved into tmRNA and the similarly-sized RNase P RNA (10Sb). The presence of pseudouridine in the mixed 10S RNA hinted that tmRNA has modified bases found also in tRNA. The similarity at the 3' end of tmRNA to the T stem-loop of tRNA was first recognized upon sequencing ssrA from Mycobacterium tuberculosis. Subsequent sequence comparison revealed the full tRNA-like domain (TLD) formed by the 5' and 3' ends of tmRNA, including the acceptor stem with elements like those in alanine tRNA that promote its aminoacylation by alanine-tRNA ligase. It also revealed differences from tRNA: the anticodon arm is missing in tmRNA, and the D arm region is a loop without base pairs.
Read more about this topic: Tm RNA
Famous quotes containing the words discovery of, discovery, early and/or work:
“The discovery of the North Pole is one of those realities which could not be avoided. It is the wages which human perseverance pays itself when it thinks that something is taking too long. The world needed a discoverer of the North Pole, and in all areas of social activity, merit was less important here than opportunity.”
—Karl Kraus (1874–1936)
“I have known no experience more distressing than the discovery that Negroes didn’t love me. Unutterable loneliness claimed me. I felt without roots, like a man without a country ...”
—Sarah Patton Boyle, U.S. civil rights activist and author. The Desegregated Heart, part 1, ch. 10 (1962)
“With boys you always know where you stand. Right in the path of a hurricane. It’s all there. The fruit flies hovering over their waste can, the hamster trying to escape to cleaner air, the bedrooms decorated in Early Bus Station Restroom.”
—Erma Bombeck (20th century)
“Human life itself may be almost pure chaos, but the work of the artist—the only thing he’s good for—is to take these handfuls of confusion and disparate things, things that seem to be irreconcilable, and put them together in a frame to give them some kind of shape and meaning. Even if it’s only his view of a meaning. That’s what he’s for—to give his view of life.”
—Katherine Anne Porter (1890–1980)