Caution
Certain ions can increase the solubility of a protein when the concentration of the ions increases, instead of decreasing the solubility of the protein. Also some ions can denature certain proteins so if assays on the function of proteins are intended then either a different ion or an alternative purification method should be used. In attempting to remove a product from water, NaCl is often used to increase the ionic strength of water, thereby increasing its polarity, and then the product is moved into the organic layer where it can be extracted.
Read more about this topic: Salting Out
Famous quotes containing the word caution:
“... your spiritual teachers caution you against enquiry—tell you not to read certain books; not to listen to certain people; to beware of profane learning; to submit your reason, and to receive their doctrines for truths. Such advice renders them suspicious counsellors.”
—Frances Wright (1795–1852)
“People who wish to numb our caution in dealing with them by means of flattery are employing a dangerous expedient, like a sleeping draught, which, if it does not put us to sleep, keeps us all the more awake.”
—Friedrich Nietzsche (1844–1900)
“I cannot say to a person who suffers injustice, “Wait.” Perhaps you can. I can’t. And having decided that I cannot urge caution I must stand with him.”
—Sandra “Casey” Cason (b. c. 1939)