Binding
The RISC with a bound siRNA recognizes complementary messenger RNA (mRNA) molecules and degrades them, resulting in substantially decreased levels of protein translation and effectively turning off the gene. It is as yet unclear how the activated RISC complex locates the mRNA targets in the cell, though it has been shown that the process can occur in situations outside of ongoing protein translation from mRNA. Endogenously expressed miRNA in metazoans is usually imperfectly complementary to a large number of nuclear genes and has a modulating effect on these genes' levels of expression via translational repression.However, in plants the process has a much greater specificity to the mRNA and usually each miRNA only binds to one mRNA. Recently identified members of RISC complex are SND1(p100 Coactivator or Tudor SN)and AEG-1(MTDH). AEG-1 and SND1 are oncogenes and regulates various gene expression.
Read more about this topic: RNA-induced Silencing Complex
Famous quotes containing the word binding:
“[Government’s] true strength consists in leaving individuals and states as much as possible to themselves—in making itself felt, not in its power, but in its beneficence, not in its control, but in its protection, not in binding the states more closely to the center, but leaving each to move unobstructed in its proper orbit.”
—Andrew Jackson (1767–1845)
“Hate traps us by binding us too tightly to our adversary.”
—Milan Kundera (b. 1929)
“With a binding like you’ve got, people are gonna want to know what’s in the book.”
—Alan Jay Lerner (1918–1986)