RDO is a three-letter initialism that stands for, among other things:
- Radio Dom Ostankino, Russian broadcaster
- Raster Document Object (.rdo), a file format used in print on demand systems manufactured by Xerox
- Rate–distortion optimization, a decision algorithm used in video compression.
- Relational Database Operator, see Oracle Rdb
- Remote Data Objects, a deprecated Microsoft technology
- Rostered Day Off
- Real Design Online, a web development company based in Portland, Oregon