Genetic Methods
Experimental analysis of proteins typically require expression and purification of proteins. Expression is achieved by manipulating DNA that encodes the protein(s) of interest. Hence protein analysis usually requires DNA methods, especially cloning. Other examples include
- conceptual translation- many proteins are never directly sequenced, but their sequence of amino acids is known by "conceptual translation" of a known mRNA sequence. See genetic code.
- site-directed mutagenesis allows new variants of proteins to be produced and tested for how structural changes alter protein function.
- insertion of protein tags such as the His-tag. See also green fluorescent protein.
- Proteins that are involved in human diseases can be identified by matching alleles to disease and other phenotypes using methods such as calculation of LOD scores.
Read more about this topic: Protein Methods
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