PRL may refer to:
- Parameter Request List, a DHCP option
- Parti Réformateur Libéral, a former Belgian political party
- Penn Eastern Rail Lines
- Pennine Radio Limited
- Physical Research Laboratory (India's national centre for research in space and allied sciences)
- Physical Review Letters (journals in physics)
- Pattern Recognition Letters (scientific journals)
- Polo Ralph Lauren - a luxury goods company by designer Ralph Lauren
- Polska Rzeczpospolita Ludowa (People's Republic of Poland)
- Preferred Roaming List - used in CDMA phones
- Professional Rugby League, the top tier, professional rugby league in Russia
- Prolactin (hormone)
- Proportional reduction in loss – a measure of reliability
- protein of regenerating liver (PRL1, 2, 3)