Powdered Milk - Use in Biotechnology

Use in Biotechnology

Fat-free powder milk is used as a saturating agent to block nonspecific binding sites on supports like blotting membranes (Nitrocellulose, PVDF, Nylon), preventing binding of further detection reagents and subsequent background. It may be referred as Blotto. The major protein of milk, casein, is responsible of most of the binding site saturation effect.