Growth of The Bacterial Culture
Plasmids are almost always purified from liquid bacteria cultures, usually E. coli, which have been transformed and isolated. Virtually all plasmid vectors in common encode one or more antibiotic resistance genes as a selectable marker for example ampicillin or kanamycin, which allows bacteria that have been successfully transformed to multiply uninhibited. Bacteria are grown under favourable conditions.
Read more about this topic: Plasmid Preparation
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—Harold MacMillan (1894–1986)
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“Why is it so difficult to see the lesbian—even when she is there, quite plainly, in front of us? In part because she has been “ghosted”Mor made to seem invisible—by culture itself.... Once the lesbian has been defined as ghostly—the better to drain her of any sensual or moral authority—she can then be exorcised.”
—Terry Castle, U.S. lesbian author. The Apparitional Lesbian, ch. 1 (1993)