Growth of The Bacterial Culture
Plasmids are almost always purified from liquid bacteria cultures, usually E. coli, which have been transformed and isolated. Virtually all plasmid vectors in common encode one or more antibiotic resistance genes as a selectable marker for example ampicillin or kanamycin, which allows bacteria that have been successfully transformed to multiply uninhibited. Bacteria are grown under favourable conditions.
Read more about this topic: Plasmid Preparation
Famous quotes containing the words growth of, growth and/or culture:
“I conceive that the leading characteristic of the nineteenth century has been the rapid growth of the scientific spirit, the consequent application of scientific methods of investigation to all the problems with which the human mind is occupied, and the correlative rejection of traditional beliefs which have proved their incompetence to bear such investigation.”
—Thomas Henry Huxley (1825–95)
“The quality of American life is an insult to the possibilities of human growth ... the pollution of American space, with gadgetry and cars and TV and box architecture, brutalizes the senses, making gray neurotics of most of us, and perverse spiritual athletes and strident self-transcenders of the best of us.”
—Susan Sontag (b. 1933)
“The hatred of the youth culture for adult society is not a disinterested judgment but a terror-ridden refusal to be hooked into the, if you will, ecological chain of breathing, growing, and dying. It is the demand, in other words, to remain children.”
—Midge Decter (b. 1927)