Growth of The Bacterial Culture
Plasmids are almost always purified from liquid bacteria cultures, usually E. coli, which have been transformed and isolated. Virtually all plasmid vectors in common encode one or more antibiotic resistance genes as a selectable marker for example ampicillin or kanamycin, which allows bacteria that have been successfully transformed to multiply uninhibited. Bacteria are grown under favourable conditions.
Read more about this topic: Plasmid Preparation
Famous quotes containing the words growth and/or culture:
“The windy springs and the blazing summers, one after another, had enriched and mellowed that flat tableland; all the human effort that had gone into it was coming back in long, sweeping lines of fertility. The changes seemed beautiful and harmonious to me; it was like watching the growth of a great man or of a great idea. I recognized every tree and sandbank and rugged draw. I found that I remembered the conformation of the land as one remembers the modelling of human faces.”
—Willa Cather (1873–1947)
“The first time many women hold their tiny babies, they are apt to feel as clumsy and incompetent as any man. The difference is that our culture tells them they’re not supposed to feel that way. Our culture assumes that they will quickly learn how to be a mother, and that assumption rubs off on most women—so they learn.”
—Pamela Patrick Novotny (20th century)