Growth of The Bacterial Culture
Plasmids are almost always purified from liquid bacteria cultures, usually E. coli, which have been transformed and isolated. Virtually all plasmid vectors in common encode one or more antibiotic resistance genes as a selectable marker for example ampicillin or kanamycin, which allows bacteria that have been successfully transformed to multiply uninhibited. Bacteria are grown under favourable conditions.
Read more about this topic: Plasmid Preparation
Famous quotes containing the words growth of the, growth of, growth and/or culture:
“I conceive that the leading characteristic of the nineteenth century has been the rapid growth of the scientific spirit, the consequent application of scientific methods of investigation to all the problems with which the human mind is occupied, and the correlative rejection of traditional beliefs which have proved their incompetence to bear such investigation.”
—Thomas Henry Huxley (1825–95)
“It is in the comprehension of the physically disabled, or disordered ... that we are behind our age.... sympathy as a fine art is backward in the growth of progress ...”
—Elizabeth Stuart Phelps (1844–1911)
“Cities force growth and make men talkative and entertaining, but they make them artificial. What possesses interest for us is the natural of each, his constitutional excellence. This is forever a surprise, engaging and lovely; we cannot be satiated with knowing it, and about it; and it is this which the conversation with Nature cherishes and guards.”
—Ralph Waldo Emerson (1803–1882)
“Popular culture is seductive; high culture is imperious.”
—Mason Cooley (b. 1927)