Growth of The Bacterial Culture
Plasmids are almost always purified from liquid bacteria cultures, usually E. coli, which have been transformed and isolated. Virtually all plasmid vectors in common encode one or more antibiotic resistance genes as a selectable marker for example ampicillin or kanamycin, which allows bacteria that have been successfully transformed to multiply uninhibited. Bacteria are grown under favourable conditions.
Read more about this topic: Plasmid Preparation
Famous quotes containing the words growth of, growth and/or culture:
“Sensuality often accelerates the growth of love so much that its roots remain weak and are easily pulled up.”
—Friedrich Nietzsche (1844–1900)
“Cities force growth and make men talkative and entertaining, but they make them artificial. What possesses interest for us is the natural of each, his constitutional excellence. This is forever a surprise, engaging and lovely; we cannot be satiated with knowing it, and about it; and it is this which the conversation with Nature cherishes and guards.”
—Ralph Waldo Emerson (1803–1882)
“The hard truth is that what may be acceptable in elite culture may not be acceptable in mass culture, that tastes which pose only innocent ethical issues as the property of a minority become corrupting when they become more established. Taste is context, and the context has changed.”
—Susan Sontag (b. 1933)