Pastorius may refer to:
- Francis Daniel Pastorius (1651 – c. 1720), leader of the first organized settlement of Germans in Pennsylvania
- Jaco Pastorius (1951–1987), jazz bassist and composer
- Jim Pastorius (James W. Pastorius) (1881–1941), professional baseball player
- Operation Pastorius, failed sabotage attack by Nazi Germany on the United States in 1942
- Pastorius Park in the Chestnut Hill neighborhood of Northwest Philadelphia, named after Francis Daniel Pastorius
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