Oklahoma Medical Research Foundation - Core Facilities

Core Facilities

OMRF maintains numerous core facilities, including:

• A 7-tesla MRI, which uses a 10,000-pound magnet to generate a magnetic field that is 140,000 times stronger than the earth’s and allows researchers to study the cells and organs of genetically engineered living mice and rats at microscopic levels without harming the animals;

• BIACore to measure affinity and binding kinetics of macromolecular interactions;

• DNA Sequencing, with the daily capacity to run 90 sequences;

• Flow Cytometry, with three instruments: the FACScan and FACSCalibur cytometers, capable of three and four color fluorescence analyses, and the MoFlo cytometer capable of high throughput cell sorting.;

• Imaging, to assist researchers with imaging needs ranging from basic light and electron microscopy to digital image processing and analysis;

• In Situ Hybridization, including tissue sectioning, slide mounting, and hybridization histochemistry;

• Mouse Genome Manipulation Facility, providing microinjection services of DNA into zygotes for the generation of transgenic mice, and of ES cells into blastocysts for the generation of knockout mice;

• Molecular Biology Resource Facility, for protein and peptide sequencing and mass spectrometry analysis; and

• Signal Transduction Core, to assist research involving intracellular Ca2+ measurements and protein-protein interactions.