Resonance Assignment
In order to analyze the nuclear magnetic resonance data, it is important to get a resonance assignment for the protein. That is to find out which chemical shift corresponds to which atom. This is typically achieved by sequential walking using information derived from several different types of NMR experiment. The exact procedure depends on whether the protein is isotopically labelled or not, since a lot of the assignment experiments depend on carbon-13 and nitrogen-15.
Read more about this topic: Nuclear Magnetic Resonance Spectroscopy Of Proteins
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“It is closing time in the gardens of the West and from now on an artist will be judged only by the resonance of his solitude or the quality of his despair.”
—Cyril Connolly (1903–1974)