Chemical and Physical Properties
Nile blue is a fluorescent dye. The fluorescence shows especially in nonpolar solvents with a high quantum yield.
The absorption and emission maxima of Nile blue are strongly dependent on pH and the solvents used:
| Solvent | Absorption λ max (nm) | Emission λ max (nm) |
|---|---|---|
| Toluene | 493 | 574 |
| Acetone | 499 | 596 |
| Dimethylformamide | 504 | 598 |
| Chloroform | 624 | 647 |
| 1-Butanol | 627 | 664 |
| 2-propanol | 627 | 665 |
| Ethanol | 628 | 667 |
| Methanol | 626 | 668 |
| Water | 635 | 674 |
| 1.0 N hydrochloric acid (pH = 1.0) | 457 | 556 |
| 0.1 N sodium hydroxide solution (pH = 11.0) | 522 | 668 |
| Ammonia water (pH = 13.0) | 524 | 668 |
The duration of Nile blue fluorescence in ethanol was measured as 1.42 ns. This is shorter than the corresponding value of Nile red with 3.65 ns. The fluorescence duration is independent on dilution in the range 10−3–10−8 mol/L.
Read more about this topic: Nile Blue
Famous quotes containing the words chemical, physical and/or properties:
“Ants are so much like human beings as to be an embarrassment. They farm fungi, raise aphids as livestock, launch armies into war, use chemical sprays to alarm and confuse enemies, capture slaves, engage in child labor, exchange information ceaselessly. They do everything but watch television.”
—Lewis Thomas (b. 1913)
“A more problematic example is the parallel between the increasingly abstract and insubstantial picture of the physical universe which modern physics has given us and the popularity of abstract and non-representational forms of art and poetry. In each case the representation of reality is increasingly removed from the picture which is immediately presented to us by our senses.”
—Harvey Brooks (b. 1915)
“The reason why men enter into society, is the preservation of their property; and the end why they choose and authorize a legislative, is, that there may be laws made, and rules set, as guards and fences to the properties of all the members of the society: to limit the power, and moderate the dominion, of every part and member of the society.”
—John Locke (1632–1704)