Synthesis
Molecular beacons are synthetic oligonucleotides whose preparation is well documented. In addition to the conventional set of nucleoside phosphoramidites, the synthesis also requires a solid support derivatized with a quencher and a phosphoramidite building block designed for the attachment of a protected fluorescent dye.
The first use of the term molecular beacons, synthesis and demonstration of function was in 1996
Read more about this topic: Molecular Beacon
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