MDT may refer to:
- Maggot debridement therapy, usage of live maggots to clean wounds with necrotic tissue
- Mean down time, the average time that a system is non-operational
- Medtronic, an American medical technology company
- Miami-Dade Transit, the public transportation agency for Miami, Florida
- Microsoft Deployment Toolkit, a tool for deployment of operating systems created by Microsoft
- Minnesota Dance Theatre, a dance school and company in Minneapolis, Minnesota
- Mobile data terminal, a type of communications equipment
- Mountain Daylight Time in North America (UTC−6)
- Harrisburg International Airport (IATA airport code), Pennsylvania, U.S.