As a surname Lockyer may refer to:
- Bill Lockyer (born 1941), American Politician
- Darren Lockyer (born 1977), Australian rugby league footballer
- Edmund Lockyer (1784-1860), British soldier and Australian explorer
- James Lockyer, Ontario, Canadian lawyer and social justice activist
- James E. Lockyer (born 1949), New Brunswick, Canadian lawyer, politician
- Norman Lockyer (1836-1920), English scientist and astronomer
- Lionel Lockyer (c. 1600-1672), a quack doctor, whose remains now lie in a tomb in Southwark Cathedral
- Malcolm Lockyer (1923-1976), film composer and conductor
- Peter Lockyer, actor and singer
- Tarkyn Lockyer (born 1979), Australian rules footballer
- Tom Lockyer (1826-1869), English cricketer
It may also refer to these places:
- Lockyer Valley, Queensland - named after Edmund Lockyer
- Lockyer Creek, a creek in the Lockyer Valley
- Electoral district of Lockyer, Queensland, Australia