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- Atlantic, Suwannee River and Gulf Railroad (not legislatively chartered)
- East Florida Railway
- Florida Midland Railway
- Florida Midland and Georgia Railroad
- Jacksonville and Atlantic Railroad
- Jacksonville, St. Augustine and Halifax River Railway (not legislatively chartered)
- Jacksonville, St. Augustine and Indian River Railway
- Monticello and Georgia Railroad
- Orange Ridge, DeLand and Atlantic Railroad
- St. Johns and Halifax Railway
- Savannah, Florida and Western Railway
- South Florida Railroad
- Thomasville, Tallahassee and Gulf Railroad
Read more about this topic: List Of Railroads Incorporated In Florida