The following is a list of cities and towns in Botswana with population of over 3,000 citizens. State capitals are shown in boldface.
| Rank | Name | District | Census District | Population 2001. | Male Population | Female Population |
|---|---|---|---|---|---|---|
| 1. | Gaborone | South-East District | Gaborone | 186,007 | 91,823 | 94,184 |
| 2. | Francistown | North-East District | Francistown | 83,023 | 40,134 | 42,889 |
| 3. | Molepolole | Kweneng District | Kweneng East | 62,739 | 28,617 | 34,122 |
| 4. | Serowe | Central District | Central Serowe/Palapye | 52,831 | 25,400 | 27,431 |
| 5. | Selibe Phikwe | Central District | Selibe Phikwe | 49,849 | 24,334 | 25,515 |
| 6. | Maun | North-West District | Ngamiland East | 49,822 | 23,714 | 26,108 |
| 7. | Kanye | Southern District | Ngwaketse | 48,143 | 22,451 | 25,692 |
| 8. | Mahalapye | Central District | Central Mahalapye | 43,538 | 21,120 | 22,418 |
| 9. | Mogoditshane | Kweneng District | Kweneng East | 40,753 | 20,972 | 19,781 |
| 10. | Mochudi | Kgatleng District | Kgatleng | 39,349 | 18,490 | 20,859 |
| 11. | Lobatse | South-East District | Lobatse | 29,689 | 14,202 | 15,487 |
| 12. | Palapye | Central District | Central Serowe/Palapye | 29,565 | 13,995 | 15,570 |
| 13. | Ramotswa | South-East District | South East | 25,738 | 12,027 | 13,711 |
| 14. | Moshupa | Southern District | Ngwaketse | 22,811 | 10,677 | 12,134 |
| 15. | Tlokweng | South-East District | South East | 22,038 | 10,568 | 11,470 |
| 16. | Bobonong | Central District | Central Bobonong | 21,020 | 9,877 | 11,143 |
| 17. | Thamaga | Kweneng District | Kweneng East | 20,527 | 9,332 | 11,195 |
| 18. | Letlhakane | Central District | Central Boteti | 19,539 | 9,848 | 9,691 |
| 19. | Tonota | Central District | Central Tutume | 18,816 | 8,745 | 10,071 |
| 20. | Mmadinare | Central District | Central Bobonong | 15,712 | 7,600 | 8,112 |
| 21. | Jwaneng | Southern District | Jwaneng | 15,179 | 7,613 | 7,566 |
| 22. | Tutume | Central District | Central Tutume | 14,707 | 6,555 | 8,152 |
| 23. | Gabane | Kweneng District | Kweneng East | 12,530 | 5,882 | 6,648 |
| 24. | Shoshong | Central District | Central Mahalapye | 11,176 | 5,521 | 5,655 |
| 25. | Ghanzi | Ghanzi District | Ghanzi | 10,795 | 5,375 | 5,420 |
| 26. | Letlhakeng | Kweneng District | Kweneng West | 9,676 | 4,685 | 4,991 |
| 27. | Orapa | Central District | Orapa | 9,151 | 4,837 | 4,314 |
| 28. | Kopong | Kweneng District | Kweneng East | 8,992 | 4,416 | 4,576 |
| 29. | Mmopane | Kweneng District | Kweneng East | 8,554 | 4,181 | 4,373 |
| 30. | Kasane | North-West District | Ngamiland West | 7,933 | 3,968 | 3,965 |
| 31. | Shakawe | North-West District | Ngamiland East | 7,874 | 3,632 | 4,242 |
| 32. | Lentsweletau | Kweneng District | Kweneng East | 7,609 | 3,815 | 3,794 |
| 33. | Molapowabojang | Southern District | Ngawaketse | 7,499 | 3,533 | 3,966 |
| 34. | Gumare | North-West District | Ngamiland West | 7,478 | 3,482 | 3,996 |
| 35. | Tshabong | Kgalagadi District | Kgalagadi South | 7,228 | 3,671 | 3,557 |
| 36. | Lerala | Central District | Central Serowe/Palapye | 6,740 | 3,107 | 3,633 |
| 37. | Mmathethe | Southern District | Borolong | 6,692 | 3,351 | 3,341 |
| 38. | Gweta | Central District | Central Tutume | 6,653 | 3,244 | 3,409 |
| 39. | Mathangwane | Central District | Central Tutume | 6,468 | 3,011 | 3,457 |
| 40. | Mmankgodi | Kweneng District | Kweneng East | 6,277 | 2,868 | 3,409 |
| 41. | Maitengwe | Central District | Central Tutume | 6,141 | 2,728 | 3,413 |
| 42. | Rakops | Central District | Central Boteti | 6,124 | 2,814 | 3,310 |
| 43. | Dukwi | Central District | Central Tutume | 5,877 | 3,220 | 2,657 |
| 44. | Mopipi | Central District | Central Boteti | 5,625 | 2,725 | 2,900 |
| 45. | Etsha | North-West District | Ngamiland West | 5,613 | 2,466 | 3,147 |
| 46. | Otse | South-East District | South East | 5,599 | 2,838 | 2,761 |
| 47. | Sefophe | Central District | Central Bobonong | 5,470 | 2,662 | 2,808 |
| 48. | Sehithwa | North-West District | Ngamiland East | 5,290 | 2,744 | 2,546 |
| 49. | Nata | Central District | Central Tutume | 5,170 | 2,381 | 2,789 |
| 50. | Tumasera/Seleka | Central District | Central Mahalapye | 5,131 | 2,401 | 2,730 |
| 51. | Moiyabana | Central District | Central Serowe/Palapye | 4,947 | 2,258 | 2,689 |
| 52. | Lotlhakane | Southern District | Ngwaketse | 4,741 | 2,371 | 2,370 |
| 53. | Metsimotlhaba | Kweneng District | Kweneng East | 4,692 | 2,162 | 2,530 |
| 54. | Bokaa | Kgatleng District | Kgatleng | 4,545 | 2,185 | 2,360 |
| 55. | Tati Siding | North-East District | North East | 4,456 | 2,242 | 2,214 |
| 56. | Tsetsebjwe | Central District | Central Bobonong | 4,396 | 2,031 | 2,365 |
| 57. | Hukuntsi | Kgalagadi District | Kgalagadi North | 4,131 | 2,052 | 2,079 |
| 58. | Kang | Kgalagadi District | Kgalagadi North | 4,124 | 2,133 | 1,991 |
| 59. | Maunatlala | Central District | Central Serowe/Palapye | 3,876 | 1,806 | 2,070 |
| 60. | Tobane | Central District | Central Bobonong | 3,718 | 1,909 | 1,809 |
| 61. | Nkange | Central District | Central Tutume | 3,707 | 1,587 | 2,120 |
| 62. | Ramokgonami | Central District | Central Mahalapye | 3,654 | 1,717 | 1,937 |
| 63. | Sebina | Central District | Central Tutume | 3,566 | 1,607 | 1,959 |
| 64. | Oodi | Kgatleng District | Kgatleng | 3,550 | 1,691 | 1,859 |
| 65. | Kumakwane | Kweneng District | Kweneng East | 3,544 | 1,668 | 1,876 |
| 66. | Manyana | Southern District | Ngwaketse | 3,488 | 1,645 | 1,843 |
| 67. | Toteng | North-West District | Ngamiland East | 3,391 | 1,826 | 1,565 |
| 68. | Shorobe | North-West District | Ngamiland East | 3,333 | 1,628 | 1,705 |
| 69. | Mandunyane | Central District | Central Tutume | 3,265 | 1,550 | 1,715 |
| 70. | Thabala | Central District | Central Serowe/Palapye | 3,255 | 1,624 | 1,631 |
| 71. | Lecheng | Central District | Central Serowe/Palapye | 3,251 | 1,536 | 1,715 |
| 72. | Shashe-Mooke | Central District | Central Tutume | 3,249 | 1,548 | 1,701 |
| 73. | Khudumelapye | Kweneng District | Kweneng West | 3,248 | 1,645 | 1,603 |
| 74. | Ranaka | Southern District | Ngwaketse | 3,124 | 1,546 | 1,578 |
| 75. | Marobela | Central District | Central Tutume | 3,114 | 1,441 | 1,673 |
| 76. | Masunga | North-East District | North East | 3,110 | 1,394 | 1,716 |
| 77. | Malolwane | Kgatleng District | Kgatleng | 3,084 | 1,559 | 1,525 |
| 78. | Borolong | Central District | Central Tutume | 3,081 | 1,423 | 1,658 |
| 79. | Nokaneng | North-West District | Ngamiland West | 3,075 | 1,564 | 1,511 |
| 80. | Chadibe | Central District | Central Mahalapye | 3,060 | 1,439 | 1,621 |
| 81. | Seronga | North-West District | Ngamiland West | 3,043 | 1,307 | 1,736 |
| 82. | Mookane | Central District | Central Mahalapye | 3,042 | 1,500 | 1,542 |
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