LFO may refer to:
- LFO (band), an English electronic music act
- LFO (group), an American three-man pop/rap group
- LFO (album), an album released by the American pop group LFO
- Light Finding Operation, a form of mecha from the anime TV series Eureka Seven
- Little Fighter Online, a fighting game for Windows
- London Festival Orchestra, the 'house orchestra' for Decca Records
- Low-frequency oscillation, an electronic signal, which is usually below 20 Hz and creates a rhythmic pulse or sweep