Monitoring Glutathione Reductase Activity
The activity of glutathione reductase is used as indicator for oxidative stress. The activity can be monitored by the NADPH consumption, with absorbance at 340 nm, or the formed GSH can be visualized by Ellman's reagent. Alternatively the activity can be measured using roGFP (redox-sensitive Green Fluorescent Protein).
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“A constant smirk upon the face, and a whiffling activity of the body, are strong indications of futility.”
—Philip Dormer Stanhope, 4th Earl Chesterfield (1694–1773)
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