Culture Method
In an air-tight gas jar the culture media, the gas-pak sachet (opened and water added) and an indicator are placed. The jar is incubated at the desired temperature. The indicator tells whether the environment was indeed oxygen free or not.
Indicator: Generally used chemical indicator for this purpose is "chemical methylene blue solution" that has never since synthesis been exposed to elemental oxygen. It is colored deep blue on oxidation in presence of atmospheric oxygen in the jar, but will become colorless when oxygen is gone, and anaerobic conditions are achieved.
Read more about this topic: Gas-pak
Famous quotes containing the words culture and/or method:
“Children became an obsessive theme in Victorian culture at the same time that they were being exploited as never before. As the horrors of life multiplied for some children, the image of childhood was increasingly exalted. Children became the last symbols of purity in a world which was seen as increasingly ugly.”
—C. John Sommerville (20th century)
“There is assuredly no more effectual method of clearing up one’s own mind on any subject than by talking it over, so to speak, with men of real power and grasp, who have considered it from a totally different point of view.”
—Thomas Henry Huxley (1825–95)