Culture Method
In an air-tight gas jar the culture media, the gas-pak sachet (opened and water added) and an indicator are placed. The jar is incubated at the desired temperature. The indicator tells whether the environment was indeed oxygen free or not.
Indicator: Generally used chemical indicator for this purpose is "chemical methylene blue solution" that has never since synthesis been exposed to elemental oxygen. It is colored deep blue on oxidation in presence of atmospheric oxygen in the jar, but will become colorless when oxygen is gone, and anaerobic conditions are achieved.
Read more about this topic: Gas-pak
Famous quotes containing the words culture and/or method:
“I know that there are many persons to whom it seems derogatory to link a body of philosophic ideas to the social life and culture of their epoch. They seem to accept a dogma of immaculate conception of philosophical systems.”
—John Dewey (1859–1952)
“Unlike Descartes, we own and use our beliefs of the moment, even in the midst of philosophizing, until by what is vaguely called scientific method we change them here and there for the better. Within our own total evolving doctrine, we can judge truth as earnestly and absolutely as can be, subject to correction, but that goes without saying.”
—Willard Van Orman Quine (b. 1908)