Culture Method
In an air-tight gas jar the culture media, the gas-pak sachet (opened and water added) and an indicator are placed. The jar is incubated at the desired temperature. The indicator tells whether the environment was indeed oxygen free or not.
Indicator: Generally used chemical indicator for this purpose is "chemical methylene blue solution" that has never since synthesis been exposed to elemental oxygen. It is colored deep blue on oxidation in presence of atmospheric oxygen in the jar, but will become colorless when oxygen is gone, and anaerobic conditions are achieved.
Read more about this topic: Gas-pak
Famous quotes containing the words culture and/or method:
“Let a man attain the highest and broadest culture that any American has possessed, then let him die by sea-storm, railroad collision, or other accident, and all America will acquiesce that the best thing has happened to him; that, after the education has gone far, such is the expensiveness of America, that the best use to put a fine person to is to drown him to save his board.”
—Ralph Waldo Emerson (1803–1882)
“Women are denied masturbation even more severely than men and that’s another method of control—they’re not taught to please themselves.... Most women—it takes them a while to warm up to the “situation” but once they get into it, I’m sure they’re going to get just as hooked as—well, everyone I know is!”
—Lydia Lunch (b. 1959)