Culture Method
In an air-tight gas jar the culture media, the gas-pak sachet (opened and water added) and an indicator are placed. The jar is incubated at the desired temperature. The indicator tells whether the environment was indeed oxygen free or not.
Indicator: Generally used chemical indicator for this purpose is "chemical methylene blue solution" that has never since synthesis been exposed to elemental oxygen. It is colored deep blue on oxidation in presence of atmospheric oxygen in the jar, but will become colorless when oxygen is gone, and anaerobic conditions are achieved.
Read more about this topic: Gas-pak
Famous quotes containing the words culture and/or method:
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—Henry David Thoreau (1817–1862)
“I am not afraid of the priests in the long-run. Scientific method is the white ant which will slowly but surely destroy their fortifications. And the importance of scientific method in modern practical life—always growing and increasing—is the guarantee for the gradual emancipation of the ignorant upper and lower classes, the former of whom especially are the strength of the priests.”
—Thomas Henry Huxley (1825–95)