Culture Method
In an air-tight gas jar the culture media, the gas-pak sachet (opened and water added) and an indicator are placed. The jar is incubated at the desired temperature. The indicator tells whether the environment was indeed oxygen free or not.
Indicator: Generally used chemical indicator for this purpose is "chemical methylene blue solution" that has never since synthesis been exposed to elemental oxygen. It is colored deep blue on oxidation in presence of atmospheric oxygen in the jar, but will become colorless when oxygen is gone, and anaerobic conditions are achieved.
Read more about this topic: Gas-pak
Famous quotes containing the words culture and/or method:
“As the twentieth century ends, commerce and culture are coming closer together. The distinction between life and art has been eroded by fifty years of enhanced communications, ever-improving reproduction technologies and increasing wealth.”
—Stephen Bayley (b. 1951)
“Protestantism has the method of Jesus with His secret too much left out of mind; Catholicism has His secret with His method too much left out of mind; neither has His unerring balance, His intuition, His sweet reasonableness. But both have hold of a great truth, and get from it a great power.”
—Matthew Arnold (1822–1888)