Frozen Sections
Small pieces of tissue (5×5×3mm) are placed in a cryoprotective embedding medium—OCT, TBS, or Cryogel—then snap frozen in isopentane cooled by liquid nitrogen. Tissue is then sectioned in a freezing microtome or cryostat. Sections are then fixed in one of the following fixatives: Absolute acetone for 10–15 minutes, 95% ethanol for 10–15 minutes or Absolute acetone 10 minutes followed by 95% ethanol 10 minutes
Read more about this topic: Fixation (histology)
Famous quotes containing the words frozen and/or sections:
“It was a good thing to have a couple of thousand people all rigid and frozen together, in the palm of one’s hand.”
—Charles Dickens (1812–1870)
“... many of the things which we deplore, the prevalence of tuberculosis, the mounting record of crime in certain sections of the country, are not due just to lack of education and to physical differences, but are due in great part to the basic fact of segregation which we have set up in this country and which warps and twists the lives not only of our Negro population, but sometimes of foreign born or even of religious groups.”
—Eleanor Roosevelt (1884–1962)