Frozen Sections
Small pieces of tissue (5×5×3mm) are placed in a cryoprotective embedding medium—OCT, TBS, or Cryogel—then snap frozen in isopentane cooled by liquid nitrogen. Tissue is then sectioned in a freezing microtome or cryostat. Sections are then fixed in one of the following fixatives: Absolute acetone for 10–15 minutes, 95% ethanol for 10–15 minutes or Absolute acetone 10 minutes followed by 95% ethanol 10 minutes
Read more about this topic: Fixation (histology)
Famous quotes containing the words frozen and/or sections:
“And if joy were not on the earth,
There were an end of change and birth,
And Earth and Heaven and Hell would die,
And in some gloomy barrow lie
Folded like a frozen fly....”
—William Butler Yeats (1865–1939)
“I have a new method of poetry. All you got to do is look over your notebooks ... or lay down on a couch, and think of anything that comes into your head, especially the miseries.... Then arrange in lines of two, three or four words each, don’t bother about sentences, in sections of two, three or four lines each.”
—Allen Ginsberg (b. 1926)