Epitope Tags
Epitopes are often used in proteomics and the study of other gene products. Using recombinant DNA techniques genetic sequences coding for epitopes that are recognized by common antibodies can be fused to the gene. Following synthesis, the resulting epitope tag allows the antibody to find the protein or other gene product enabling lab techniques for localization, purification, and further molecular characterization. Common epitopes used for this purpose are Myc-tag, HA-tag, FLAG-tag, GST-tag, 6xHis and OLLAS. Peptides can also be bound by proteins that form covalent bonds to the peptide, allowing irreversible immobilization.
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Famous quotes containing the word tags:
“Worry and brown desk
Stain it by infusion. There aren’t enough tags at the end,
And the grove is blind, blossoming, but we are too porous to hear it.”
—John Ashbery (b. 1927)