EOT is an abbreviation for:
- Embedded OpenType, a font file format
- End-of-tape, a term in magnetic tape data storage
- End Of Train device, see Flashing rear-end device, as used by the railroad industry
- End-of-transmission character, a transmission control character in telecommunication
- Engine order telegraph, a ship or submarine's speed control
- Equation of time, a timekeeping correction
- Equivalent oxide thickness, a term for the oxide in an electrical transistor
- Executive Office of Transportation (EOT), a division of the Massachusetts state government in-charge of Transportation
- Extraordinary optical transmission, an optical phenomenon
- Greek National Tourism Organisation