EBI can refer to:
- École de Biologie Industrielle, a French engineering school
- Effective buying income
- Elim Bible Institute, a college
- Encyclopædia Britannica Inc., a publisher
- Energy Biosciences Institute, a research program that will explore how bioscience can be used to increase energy production and reduce the impact of energy consumption on the environment
- European Bioinformatics Institute, a centre for research
- Evangelical Bible Institute, a school
- External Bus Interface, a computer bus
- Extra Beveiligde Inrichting, a Dutch maximum security prison in Vught