Despina is a given name for girls, meaning 'lady' in Greek. It may refer to:
- Despina Delios
- Despina Pavlou
- Despina Vandi
It may also refer to:
- Despoina, a figure in Greek mythology
- Maria Despina
- Despina (moon), a moon of Neptune
- Despina, a character in Mozart's opera Così fan tutte
- "Despoina", a song by Nikos Karvelas