Examples of Dark Quenchers
- Dabsyl (dimethylaminoazobenzenesulfonic acid) absorbs in the green spectrum and is often used with fluorescein. (Dabsyl has a nearly identical absorption, but has a sulfonyl chloride to form more stable conjugates, instead of a succinimidyl ester).
- Black Hole Quenchers are capable of quenching across the entire visible spectrum.
- Qxl quenchers span the full visible spectrum.
- Iowa black FQ absorbs in the green-yellow part of the spectrum.
- Iowa black RQ blocks in the orange-red part of the spectrum.
- IRDye QC-1 quenches dyes from the visible to the near-infrared range (500-900 nm).
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