Advantages
During the separation, the cell only needs to be suspended in a buffer solution and enter a centrifuge, the whole processes does not involve any chemical (e.g. staining) and physical (e.g. attachment of antibody, lyses of cell membrane) effect on the cells, so the cell will remain unchanged before and after the separation. Because of this, the collected cells can be used for further experiment or further separation by other techniques. Finally the CCE rely on centrifugal force and the counter flow drag force to separate the cells, so the speed of separation is fast. In summary:
- Minimum effect on the cells
- High recovery viability
- Separated cells can be used further
- Rapid
Read more about this topic: Counterflow Centrifugation Elutriation
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