History
The 3C methodology was originally developed by Dekker in the Kleckner lab in 2002 at Harvard University and it aimed at identifying, locating and mapping physical interactions between genetic elements located throughout the human genome. This technology would give beneficial insights into the complex interplay of genetic factors that contribute to such debilitating disorders such as cancer, Duchenne muscular dystrophy (DMD), Rett syndrome and Alzheimer's disease.
3C is based on proximity ligation, which had been used previously to determine circularization frequencies of DNA in solution, and the effect of protein-mediated DNA bending on circularization. Seyfred and colleagues developed proximity ligation in nuclei: restriction enzyme digestion of unfixed nuclei and ligation in situ without diluting the chromatin, which they termed the "Nuclear Ligation Assay" (Cullen et al. Science. 1993 Jul 9;261(5118):203-6; Gothard LQ et al. Mol Endocrinol. 1996 Feb;10(2):185-95).
Read more about this topic: Chromosome Conformation Capture
Famous quotes containing the word history:
“If you look at the 150 years of modern China’s history since the Opium Wars, then you can’t avoid the conclusion that the last 15 years are the best 15 years in China’s modern history.”
—J. Stapleton Roy (b. 1935)
“Classes struggle, some classes triumph, others are eliminated. Such is history; such is the history of civilization for thousands of years.”
—Mao Zedong (1893–1976)