Charlene may refer to:
- Charlene (given name), people with the given name Charlene
- "Charlene" (song) by Anthony Hamilton
- "Charlene (I'm Right Behind You)", a song by Stephen And The Colberts
- Charlene (band), a shoegazing band from Allston, Massachusetts
- Charlene, the rifle of character Gomer Pyle in Stanley Kubrick's film Full Metal Jacket