Biot or BIOT may refer to:
- Biot, Alpes-Maritimes, a commune in France
- Biot (crater), a lunar crater
- British Indian Ocean Territory
- Camille Biot (1850-1918), French physician
- Biot's breathing refers to an abnormal pattern of breathing characterized by groups of quick, shallow inspirations followed by regular or irregular periods of apnea.
- Jean-Baptiste Biot (1774–1862), French physicist and mathematician
- Biot number, a dimensionless number used in heat transfer calculations
- Another name for the abampere, a unit of current, named after him
- Maurice Anthony Biot (1905–1985), Belgian-American physicist
- Biological robot, a term used in the novel Rendezvous with Rama and its sequels, and later adopted by the authors of The New Jedi Order; see also Cyborg
- Biological Optical Transputer Systems, robots in the Phantom 2040 animated series
- Biot, a fictional world in the DC Comics universe; see Manhunters (comics)