Astudillo is a Spanish surname, it could refer to:
- MartÃn Astudillo (1977-), Argentine footballer
- Pedro "Pete" Astudillo, Texan songwriter
- Luciano Astudillo, a Swedish parliament member
- Cristian Dale Astudillo, a Philippine basketball player.
Astudillo is also the name of a Spanish city it could refer to:
- Astudillo, Palencia, a municipality in the Spanish autonomous community of Castilla y Leon part of the Province of Palencia