Method
The blood is mixed with a small amount of tap water to cause hemolysis. The sample is next centrifuged for several minutes. The pink hemoglobin-containing supernatant is then mixed with 1 mL of 1% NaOH for each 5 mL of supernatant. The color of the fluid is assessed after 2 minutes. Fetal hemoglobin will stay pink and adult hemoglobin will turn yellow-brown since adult hemoglobin will convert to hematin which has a hydroxide ligand.
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Famous quotes containing the word method:
“Protestantism has the method of Jesus with His secret too much left out of mind; Catholicism has His secret with His method too much left out of mind; neither has His unerring balance, His intuition, His sweet reasonableness. But both have hold of a great truth, and get from it a great power.”
—Matthew Arnold (1822–1888)
“There is assuredly no more effectual method of clearing up one’s own mind on any subject than by talking it over, so to speak, with men of real power and grasp, who have considered it from a totally different point of view.”
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