Method
The blood is mixed with a small amount of tap water to cause hemolysis. The sample is next centrifuged for several minutes. The pink hemoglobin-containing supernatant is then mixed with 1 mL of 1% NaOH for each 5 mL of supernatant. The color of the fluid is assessed after 2 minutes. Fetal hemoglobin will stay pink and adult hemoglobin will turn yellow-brown since adult hemoglobin will convert to hematin which has a hydroxide ligand.
Read more about this topic: Apt Test
Famous quotes containing the word method:
“I know no method to secure the repeal of bad or obnoxious laws so effective as their stringent execution.”
—Ulysses S. Grant (1822–1885)
“in the absence of feet, “a method of conclusions”;
“a knowledge of principles,”
in the curious phenomenon of your occipital horn.”
—Marianne Moore (1887–1972)
“Too poor for a bribe, and too proud to importune,
He had not the method of making a fortune.”
—Thomas Gray (1716–1771)