Method
The blood is mixed with a small amount of tap water to cause hemolysis. The sample is next centrifuged for several minutes. The pink hemoglobin-containing supernatant is then mixed with 1 mL of 1% NaOH for each 5 mL of supernatant. The color of the fluid is assessed after 2 minutes. Fetal hemoglobin will stay pink and adult hemoglobin will turn yellow-brown since adult hemoglobin will convert to hematin which has a hydroxide ligand.
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Famous quotes containing the word method:
““English! they are barbarians; they don’t believe in the great God.” I told him, “Excuse me, Sir. We do believe in God, and in Jesus Christ too.” “Um,” says he, “and in the Pope?” “No.” “And why?” This was a puzzling question in these circumstances.... I thought I would try a method of my own, and very gravely replied, “Because we are too far off.” A very new argument against the universal infallibility of the Pope.”
—James Boswell (1740–1795)
“In child rearing it would unquestionably be easier if a child were to do something because we say so. The authoritarian method does expedite things, but it does not produce independent functioning. If a child has not mastered the underlying principles of human interactions and merely conforms out of coercion or conditioning, he has no tools to use, no resources to apply in the next situation that confronts him.”
—Elaine Heffner (20th century)
““I have usually found that there was method in his madness.”
“Some folk might say there was madness in his method.””
—Sir Arthur Conan Doyle (1859–1930)