Method
The blood is mixed with a small amount of tap water to cause hemolysis. The sample is next centrifuged for several minutes. The pink hemoglobin-containing supernatant is then mixed with 1 mL of 1% NaOH for each 5 mL of supernatant. The color of the fluid is assessed after 2 minutes. Fetal hemoglobin will stay pink and adult hemoglobin will turn yellow-brown since adult hemoglobin will convert to hematin which has a hydroxide ligand.
Read more about this topic: Apt Test
Famous quotes containing the word method:
“Direct action ... is the logical, consistent method of Anarchism.”
—Emma Goldman (1869–1940)
“Argument is conclusive ... but ... it does not remove doubt, so that the mind may rest in the sure knowledge of the truth, unless it finds it by the method of experiment.... For if any man who never saw fire proved by satisfactory arguments that fire burns ... his hearer’s mind would never be satisfied, nor would he avoid the fire until he put his hand in it ... that he might learn by experiment what argument taught.”
—Roger Bacon (c. 1214–1294)
“It is to be lamented that the principle of national has had very little nourishment in our country, and, instead, has given place to sectional or state partialities. What more promising method for remedying this defect than by uniting American women of every state and every section in a common effort for our whole country.”
—Catherine E. Beecher (1800–1878)