Method
The blood is mixed with a small amount of tap water to cause hemolysis. The sample is next centrifuged for several minutes. The pink hemoglobin-containing supernatant is then mixed with 1 mL of 1% NaOH for each 5 mL of supernatant. The color of the fluid is assessed after 2 minutes. Fetal hemoglobin will stay pink and adult hemoglobin will turn yellow-brown since adult hemoglobin will convert to hematin which has a hydroxide ligand.
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“The good husband finds method as efficient in the packing of fire-wood in a shed, or in the harvesting of fruits in the cellar, as in Peninsular campaigns or the files of the Department of State.”
—Ralph Waldo Emerson (1803–1882)
“You know, I have a method all my own. If you’ll notice, the coat came first, then the tie, then the shirt. Now, according to Hoyle, after that the pants should be next. There’s where I’m different. I go for the shoes next. First the right, then the left. After that, it’s every man for himself.”
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—Woodrow Wilson (1856–1924)