Method
The blood is mixed with a small amount of tap water to cause hemolysis. The sample is next centrifuged for several minutes. The pink hemoglobin-containing supernatant is then mixed with 1 mL of 1% NaOH for each 5 mL of supernatant. The color of the fluid is assessed after 2 minutes. Fetal hemoglobin will stay pink and adult hemoglobin will turn yellow-brown since adult hemoglobin will convert to hematin which has a hydroxide ligand.
Read more about this topic: Apt Test
Famous quotes containing the word method:
“Government by average opinion is merely a circuitous method of going to the devil; those who profess to lead but in fact slavishly follow this average opinion are simply the fastest runners and the loudest squeakers of the herd which is rushing blindly down to its destruction.”
—Thomas Henry Huxley (1825–95)
“... [a] girl one day flared out and told the principal “the only mission opening before a girl in his school was to marry one of those candidates [for the ministry].” He said he didn’t know but it was. And when at last that same girl announced her desire and intention to go to college it was received with about the same incredulity and dismay as if a brass button on one of those candidate’s coats had propounded a new method for squaring the circle or trisecting the arc.”
—Anna Julia Cooper (1859–1964)
“The method of painting is the natural growth out of a need. I want to express my feelings rather than illustrate them. Technique is just a means of arriving at a statement.... I can control the flow of paint: there is no accident, just as there is no beginning and no end.”
—Jackson Pollock (1912–1956)