Method
The blood is mixed with a small amount of tap water to cause hemolysis. The sample is next centrifuged for several minutes. The pink hemoglobin-containing supernatant is then mixed with 1 mL of 1% NaOH for each 5 mL of supernatant. The color of the fluid is assessed after 2 minutes. Fetal hemoglobin will stay pink and adult hemoglobin will turn yellow-brown since adult hemoglobin will convert to hematin which has a hydroxide ligand.
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Famous quotes containing the word method:
“Unlike Descartes, we own and use our beliefs of the moment, even in the midst of philosophizing, until by what is vaguely called scientific method we change them here and there for the better. Within our own total evolving doctrine, we can judge truth as earnestly and absolutely as can be, subject to correction, but that goes without saying.”
—Willard Van Orman Quine (b. 1908)
“You know, I have a method all my own. If you’ll notice, the coat came first, then the tie, then the shirt. Now, according to Hoyle, after that the pants should be next. There’s where I’m different. I go for the shoes next. First the right, then the left. After that, it’s every man for himself.”
—Robert Riskin (1897–1955)
“I do not know a method of drawing up an indictment against a whole people.”
—Edmund Burke (1729–1797)