Method
The blood is mixed with a small amount of tap water to cause hemolysis. The sample is next centrifuged for several minutes. The pink hemoglobin-containing supernatant is then mixed with 1 mL of 1% NaOH for each 5 mL of supernatant. The color of the fluid is assessed after 2 minutes. Fetal hemoglobin will stay pink and adult hemoglobin will turn yellow-brown since adult hemoglobin will convert to hematin which has a hydroxide ligand.
Read more about this topic: Apt Test
Famous quotes containing the word method:
“Women are denied masturbation even more severely than men and that’s another method of control—they’re not taught to please themselves.... Most women—it takes them a while to warm up to the “situation” but once they get into it, I’m sure they’re going to get just as hooked as—well, everyone I know is!”
—Lydia Lunch (b. 1959)
“One of the grotesqueries of present-day American life is the amount of reasoning that goes into displaying the wisdom secreted in bad movies while proving that modern art is meaningless.... They have put into practise the notion that a bad art work cleverly interpreted according to some obscure Method is more rewarding than a masterpiece wrapped in silence.”
—Harold Rosenberg (1906–1978)
“Traditional scientific method has always been at the very best 20-20 hindsight. It’s good for seeing where you’ve been. It’s good for testing the truth of what you think you know, but it can’t tell you where you ought to go.”
—Robert M. Pirsig (b. 1928)