Method
The blood is mixed with a small amount of tap water to cause hemolysis. The sample is next centrifuged for several minutes. The pink hemoglobin-containing supernatant is then mixed with 1 mL of 1% NaOH for each 5 mL of supernatant. The color of the fluid is assessed after 2 minutes. Fetal hemoglobin will stay pink and adult hemoglobin will turn yellow-brown since adult hemoglobin will convert to hematin which has a hydroxide ligand.
Read more about this topic: Apt Test
Famous quotes containing the word method:
“As a science of the unconscious it is a therapeutic method, in the grand style, a method overarching the individual case. Call this, if you choose, a poet’s utopia.”
—Thomas Mann (1875–1955)
“Women stand related to beautiful nature around us, and the enamoured youth mixes their form with moon and stars, with woods and waters, and the pomp of summer. They heal us of awkwardness by their words and looks. We observe their intellectual influence on the most serious student. They refine and clear his mind: teach him to put a pleasing method into what is dry and difficult.”
—Ralph Waldo Emerson (1803–1882)
“Direct action ... is the logical, consistent method of Anarchism.”
—Emma Goldman (1869–1940)