Method
The blood is mixed with a small amount of tap water to cause hemolysis. The sample is next centrifuged for several minutes. The pink hemoglobin-containing supernatant is then mixed with 1 mL of 1% NaOH for each 5 mL of supernatant. The color of the fluid is assessed after 2 minutes. Fetal hemoglobin will stay pink and adult hemoglobin will turn yellow-brown since adult hemoglobin will convert to hematin which has a hydroxide ligand.
Read more about this topic: Apt Test
Famous quotes containing the word method:
“The insidiousness of science lies in its claim to be not a subject, but a method. You could ignore a subject; no subject is all-inclusive. But a method can plausibly be applied to anything within the field of consciousness.”
—Katharine Fullerton Gerould (1879–1944)
“Frankly, I adore your catchy slogan, “Adoption, not Abortion,” although no one has been able to figure out, even with expert counseling, how to use adoption as a method of birth control, or at what time of the month it is most effective.”
—Barbara Ehrenreich (b. 1941)
“Direct action ... is the logical, consistent method of Anarchism.”
—Emma Goldman (1869–1940)