Method
The blood is mixed with a small amount of tap water to cause hemolysis. The sample is next centrifuged for several minutes. The pink hemoglobin-containing supernatant is then mixed with 1 mL of 1% NaOH for each 5 mL of supernatant. The color of the fluid is assessed after 2 minutes. Fetal hemoglobin will stay pink and adult hemoglobin will turn yellow-brown since adult hemoglobin will convert to hematin which has a hydroxide ligand.
Read more about this topic: Apt Test
Famous quotes containing the word method:
“There is assuredly no more effectual method of clearing up one’s own mind on any subject than by talking it over, so to speak, with men of real power and grasp, who have considered it from a totally different point of view.”
—Thomas Henry Huxley (1825–95)
“Methinks the human method of expression by sound of tongue is very elementary, & ought to be substituted for some ingenious invention which should be able to give vent to at least six coherent sentences at once.”
—Virginia Woolf (1882–1941)
“Too poor for a bribe, and too proud to importune,
He had not the method of making a fortune.”
—Thomas Gray (1716–1771)