Signaling
The nerve secretes agrin, resulting in phosphorylation of the MuSK receptor.
It seems that the MuSK receptor recruits casein kinase 2, which is required for clustering.
A protein called rapsyn is then recruited to the primary MuSK scaffold, to induce the additional clustering of acetylcholine receptors (AChR). This is thought of as the secondary scaffold. A protein called Dok-7 has shown to be additionally required for the formation of the secondary scaffold; it is apparently recruited after MuSK phosphorylation and before acetylcholine receptors are clustered.
Read more about this topic: Agrin